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Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture Scientia Agricola
Pompeu,Georgia Bertoni; Gratão,Priscila Lupino; Vitorello,Victor Alexandre; Azevedo,Ricardo Antunes.
Exposure to nickel (Ni) at high concentrations can lead to production of reactive oxygen species (ROS) resulting in oxidative damage at the cellular level. We investigated the antioxidative responses of Nicotiana tabacum cv BY-2 cell suspension to Ni stress (0.075 and 0.75 mM NiCl2) over a 72 h period with special attention to potential alterations in isoenzymes of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR). Two main SOD isoenzymes were observed, a Mn-SOD (band I) and a Fe-SOD (band II), as well as one CAT isoenzyme and four GR isoenzymes. Activity staining analysis revealed that CAT activity plays a major role in the early response to Ni-induced oxidative stress, particularly when the Ni concentration used was low, whilst a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant enzymes; Catalase; Nickel; Superoxide dismutase.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500015
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Enzymatic hydrolysis of sugarcane bagasse pretreated with acid or alkali BABT
Pietrobon,Vivian Cristina; Monteiro,Regina Teresa Rosim; Pompeu,Georgia Bertoni; Borges,Eduardo Poggi e; Lopes,Mario Lucio; Amorim,Henrique Vianna de; Cruz,Sandra Helena da; Viégas,Ellen Karine Diniz.
The aim of this study was to evaluate the performance of enzymatic hydrolysis of acid or alkali pretreated sugarcane bagasse for the production of fermentable sugars. The first step consisted of selection of commercial enzymes presenting the highest cellulolytic activities. After selection of four enzymes: HPL, CL, P1 and P4, their performances were tested in the bagasse pretreated with acid and alkali. The sugar content of the hydrolysates was analyzed by anion exchange liquid chromatography. Data showed that the joint action of 0.5% acid pretreatment, 121ºC, 30 minutes and enzyme CL provides the best results, 67.25 g of hexose and 148.13g of pentose per kg of dry bagasse.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bagasse sugarcane; Hydrolysis; Cellulolytic enzymes; HPAEC.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200002
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Potential of hydrogen (pH) differentially modulates cadmium stress response in abscisic acid-deficient sitiens tomato mutant Bragantia
Pompeu,Georgia Bertoni; Ambrosano,Guilherme Bovi; Vilhena,Milca Bartz; Carvalho,Rogério Falleiros; Gratão,Priscila Lupino; Andrino,Felipe; Lira,Simone Possedente de; Azevedo,Ricardo Antunes.
ABSTRACT Uptake of nutrients and cadmium (Cd) are dependent upon many factors, including plant species, ions concentration and pH. Tolerance to Cd-induced oxidative stress can be modulated by phytohormones such as abscisic acid (ABA), which induce the production of reactive oxygen species, activating proteins and enzymes involved in stress response and possibly stress tolerance. The present study aimed to evaluate the biochemical variations induced by Cd in ABA-deficient sitiens tomato mutant (sit) and its wild-type counterpart, Micro-Tom (MT), grown at different pH conditions. The plants were cultivated in nutrient solution (pH 5, 6 and 7; 20-days) and were then further grown over a 48-h period in 0 or 50 ?M CdCl2 at pH 6. Before Cd addition, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant enzymes; Micro-Tom; Mineral nutrition; Oxidative stress; Solanum lycopersicum.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000300317
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Tangerineiras como porta-enxertos para laranjeira 'Pêra' Ciência e Agrotecnologia
Pompeu Junior,Jorgino; Blumer,Silvia; Pompeu,Georgia Bertoni.
Laranjeiras 'Pêra' enxertadas em 11 cultivares ou híbridos de tangerineiras foram plantadas em 1988 em Itirapina (SP), num Latossolo Vermelho-Amarelo textura arenosa, no espaçamento 4,25m x 7,5m e conduzidas sem irrigação sob clima Cwa. Tristeza e declínio são endêmicos na região. As tangerineiras 'África do Sul', 'Xienkhouanga', 'Empress', 'Cleópatra', 'Wildt', 117.477 e 'Sul da África', sem diferirem entre si, induziram, em ordem decrescente, as maiores produções de frutos às laranjeiras 'Pêra', no total das quatro primeiras colheitas do experimento. Num total de nove colheitas, as cultivares Xienkhouanga, África do Sul, 117.477, Cleópatra, Empress, Wildt e Szinkon x Tizon, sem diferirem entre si, proporcionaram, em ordem decrescente, as maiores...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus reshni; C. reticulata; Declínio; Tristeza.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000400028
Registros recuperados: 4
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